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Hence, both capped and 5' monophosphate RNAs will be cloned.","rnaextract":"shortTotal","rnaextract description":"Rna shorter than 200 nt that has not been seperated based on Poly Adenalyation","biorep":"005WC","labexpid":"LID20905","readtype":"1x36","readtype description":"Single 36 nt reads","replicate":"1","original_filename":"GSM897073_hg19_wgEncodeCshlShortRnaSeqH1hescCellShorttotalTapPlusRawRep1.bigWig"},"geolst":["GSM897073"]},{"type":"bigWig","name":"Stanford ChipSeq H1-hESC SIN3A","url":"http://egg.wustl.edu/d/hg19/GSM935289_1.bigWig","public":true,"mode":1,"qtc":{"anglescale":1,"pr":0,"pg":0,"pb":230,"nr":255,"ng":0,"nb":0,"pth":"#ffff00","nth":"#800000","thtype":0,"thmin":0,"thmax":10,"thpercentile":90,"height":10,"summeth":1},"annotation":["11101","24638","30018"],"details":{"lab":"Stanford","lab description":"Snyder - Stanford University","datatype":"ChipSeq","datatype description":"Chromatin IP Sequencing","cell":"H1-hESC","cell organism":"human","cell description":"embryonic stem cells","cell karyotype":"normal","cell lineage":"inner cell mass","cell sex":"M","treatment":"None","treatment description":"No special treatment or protocol applies","antibody":"SIN3A_(NB600-1263)","antibody antibodydescription":"Rabbit polyclonal, Immunogen: Synthetic peptide: MKRRLDDQESDVYAAQQRR, corresponding to amino acids 1-19 of Mouse mSin3A. 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Phosphorylation of NRF1 enhances DNA binding.","antibody vendorname":"Abcam","antibody vendorid":"ab55744","control":"IgG-rab","control description":"Input signal from Normal Rabbit IgG ChIP-seq.","controlid":"wgEncodeEH001834","replicate":"1","original_filename":"GSM935308_hg19_wgEncodeSydhTfbsH1hescNrf1IggrabSig.bigWig"},"geolst":["GSM935308"]},{"type":"bigWig","name":"RIKEN Cage H1-hESC cytosol","url":"http://egg.wustl.edu/d/hg19/GSM849356_2.bigWig","public":true,"mode":1,"qtc":{"anglescale":1,"pr":0,"pg":0,"pb":230,"nr":255,"ng":0,"nb":0,"pth":"#ffff00","nth":"#800000","thtype":0,"thmin":0,"thmax":10,"thpercentile":90,"height":10,"summeth":1},"annotation":["11101","25004","30011"],"details":{"lab":"RIKEN","lab description":"Carninci - RIKEN Omics Science Center","datatype":"Cage","datatype description":"CAGE","cell":"H1-hESC","cell organism":"human","cell description":"embryonic stem cells","cell karyotype":"normal","cell lineage":"inner cell mass","cell sex":"M","localization":"cytosol","localization description":"The fluid between the cells outer membrane and the nucleus","rnaextract":"longPolyA","rnaextract description":"Poly(A)+ RNA longer than 200 nt","biorep":"006C","labexpid":"J86","replicate":"2","replicate description":"tier 2","original_filename":"GSM849356_hg19_wgEncodeRikenCageH1hescCytosolPapPlusSignalRep2.bigWig"},"geolst":["GSM849356"]},{"type":"bigWig","name":"Broad ChipSeq H1-hESC SAP30","url":"http://egg.wustl.edu/d/hg19/GSM1003572_1.bigWig","public":true,"mode":1,"qtc":{"anglescale":1,"pr":0,"pg":0,"pb":230,"nr":255,"ng":0,"nb":0,"pth":"#ffff00","nth":"#800000","thtype":0,"thmin":0,"thmax":10,"thpercentile":90,"height":10,"summeth":1},"annotation":["11101","22053","30003"],"details":{"datatype":"ChipSeq","datatype description":"Chromatin IP Sequencing","antibody antibodydescription":"This SAP30 antibody (pAb) was raised against recombinant full length human SAP30 protein. 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(provided by RefSeq)","antibody vendorname":"Santa Cruz Biotechnology","antibody vendorid":"sc-643","controlid":"SL6018","labexpid":"SL6005,SL5690","softwareversion":"MACS","cell sex":"M","antibody":"SP2_(SC-643)","treatment":"None","treatment description":"No special treatment or protocol applies","protocol":"v042211.1","protocol description":"Faster ChIP protocol & AMpure XP size selection for ChIP-seq (Myers)","replicate":"1,2","original_filename":"GSM1010776_hg19_wgEncodeHaibTfbsH1hescSp2V0422111RawRep2.bigWig"},"geolst":["GSM1010776"]},{"type":"bigWig","name":"HudsonAlpha ChipSeq H1-hESC SP2","url":"http://egg.wustl.edu/d/hg19/GSM1010776_1.bigWig","public":true,"mode":1,"qtc":{"anglescale":1,"pr":0,"pg":0,"pb":230,"nr":255,"ng":0,"nb":0,"pth":"#ffff00","nth":"#800000","thtype":0,"thmin":0,"thmax":10,"thpercentile":90,"height":10,"summeth":1},"annotation":["11101","24043","30015"],"details":{"datatype":"ChipSeq","datatype description":"Chromatin IP Sequencing","antibody antibodydescription":"Rabbit polyclonal IgG, epitope mapping near the N-terminus of SP2 of human origin. 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(provided by RefSeq)","antibody vendorname":"Santa Cruz Biotechnology","antibody vendorid":"sc-643","controlid":"SL6018","labexpid":"SL6005,SL5690","softwareversion":"MACS","cell sex":"M","antibody":"SP2_(SC-643)","treatment":"None","treatment description":"No special treatment or protocol applies","protocol":"v042211.1","protocol description":"Faster ChIP protocol & AMpure XP size selection for ChIP-seq (Myers)","replicate":"1,2","original_filename":"GSM1010776_hg19_wgEncodeHaibTfbsH1hescSp2V0422111RawRep1.bigWig"},"geolst":["GSM1010776"]},{"type":"bigWig","name":"HudsonAlpha ChipSeq H1-hESC SIX5","url":"http://egg.wustl.edu/d/hg19/GSM803405_2.bigWig","public":true,"mode":1,"qtc":{"anglescale":1,"pr":0,"pg":0,"pb":230,"nr":255,"ng":0,"nb":0,"pth":"#ffff00","nth":"#800000","thtype":0,"thmin":0,"thmax":10,"thpercentile":90,"height":10,"summeth":1},"annotation":["11101","24016","30015"],"details":{"lab":"HudsonAlpha","lab description":"Myers - Hudson Alpha Institute for Biotechnology","datatype":"ChipSeq","datatype description":"ChIP-Seq","cell":"H1-hESC","cell organism":"human","cell description":"embryonic stem cells","cell karyotype":"normal","cell lineage":"inner cell mass","cell sex":"M","antibody":"SIX5","antibody antibodydescription":"Six5 (K-20) Goat polyclonal IgG, 200?g/ml. 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Recommended for detection of Sp1 of m, r and h origin by WB, IP, IF and ELISA.","antibody targetdescription":"SP1 is a transcription factor that binds to GC box promoters elements and selectively activates mRNA synthesis from genes that contain functional recognition sites. It can interact with G/C-rich motifs from the serotonin receptor promoter.","antibody vendorname":"Santa Cruz Biotech","antibody vendorid":"sc-17824","treatment":"None","treatment description":"No special treatment or protocol applies","protocol":"PCR1x","protocol description":"1-cycle of PCR (Myers)","controlid":"SL1398","labexpid":"SL1166","replicate":"1","replicate description":"tier 1","original_filename":"GSM803377_hg19_wgEncodeHaibTfbsH1hescSp1Pcr1xRawRep1.bigWig"},"geolst":["GSM803377"]},{"type":"bigWig","name":"H1 SUZ12","url":"http://egg.wustl.edu/d/hg19/GSM831042_1.bigWig","public":true,"mode":1,"qtc":{"anglescale":1,"pr":0,"pg":0,"pb":230,"nr":255,"ng":0,"nb":0,"pth":"#ffff00","nth":"#800000","thtype":0,"thmin":0,"thmax":10,"thpercentile":90,"height":10,"summeth":1},"annotation":["11101","22046","30003"],"details":{"cell type":"H1 ES cells were grown in TeSR media on Matrigel (Cellular Dynamics)","chip antibody":"Active motif,39357","original_filename":"GSM831042_H1_SUZ12.bw"},"geolst":["GSM831042"]},{"type":"bigWig","name":"Broad ChipSeq H1-hESC H2A.Z","url":"http://egg.wustl.edu/d/hg19/GSM1003579_1.bigWig","public":true,"mode":1,"qtc":{"anglescale":1,"pr":51,"pg":102,"pb":190,"nr":255,"ng":0,"nb":0,"pth":"#ffff00","nth":"#800000","thtype":0,"thmin":0,"thmax":10,"thpercentile":90,"height":10,"summeth":1},"annotation":["11101","22028","30003"],"details":{"datatype":"ChipSeq","datatype description":"Chromatin IP Sequencing","antibody antibodydescription":"Rabbit polyclonal antibody raised against a peptide corresponding to the C-terminus of H2AZ. Antibody Target: H2AZ","antibody targetdescription":"H2A.Z is a sequence variant of Histone H2A.","antibody vendorname":"Upstate (Millipore)","antibody vendorid":"07-594","controlid":"H1-hESC/Input/std","replicate":"1,2","softwareversion":"ScriptureVPaperR3","cell sex":"M","antibody":"H2A.Z","treatment":"None","treatment description":"No special treatment or protocol applies","control":"std","control description":"Standard input signal for most experiments.","original_filename":"GSM1003579_hg19_wgEncodeBroadHistoneH1hescH2azStdSig.bigWig"},"geolst":["GSM1003579"]},{"type":"bigWig","name":"HudsonAlpha ChipSeq H1-hESC Max","url":"http://egg.wustl.edu/d/hg19/GSM1010898_2.bigWig","public":true,"mode":1,"qtc":{"anglescale":1,"pr":0,"pg":0,"pb":230,"nr":255,"ng":0,"nb":0,"pth":"#ffff00","nth":"#800000","thtype":0,"thmin":0,"thmax":10,"thpercentile":90,"height":10,"summeth":1},"annotation":["11101","24066","30015"],"details":{"datatype":"ChipSeq","datatype description":"Chromatin IP Sequencing","antibody antibodydescription":"Rabbit polyclonal IgG, epitope mapping at the C-terminus of Max of human origin. Antibody Target: Max","antibody targetdescription":"The protein encoded by this gene is a member of the basic helix-loop-helix leucine zipper (bHLHZ) family of transcription factors. It is able to form homodimers and heterodimers with other family members, which include Mad, Mxi1 and Myc. Myc is an oncoprotein implicated in cell proliferation, differentiation and apoptosis. The homodimers and heterodimers compete for a common DNA target site (the E box) and rearrangement among these dimer forms provides a complex system of transcriptional regulation. 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Several alternatively spliced transcript variants of this gene have been described, but the full-length nature of some of these variants has not been determined.","antibody vendorname":"Santa Cruz Biotech","antibody vendorid":"sc-357","treatment":"None","treatment description":"No special treatment or protocol applies","protocol":"PCR1x","protocol description":"1-cycle of PCR (Myers)","controlid":"SL1398","labexpid":"SL1158","replicate":"1","replicate description":"tier 1","original_filename":"GSM803427_hg19_wgEncodeHaibTfbsH1hescTcf12Pcr1xRawRep1.bigWig"},"geolst":["GSM803427"]},{"type":"bigWig","name":"Bernstein H1-hESC H3K9ac","url":"http://egg.wustl.edu/d/hg19/GSM733773_1.bigWig","public":true,"mode":1,"qtc":{"anglescale":1,"pr":46,"pg":92,"pb":0,"nr":255,"ng":0,"nb":0,"pth":"#ffff00","nth":"#800000","thtype":0,"thmin":0,"thmax":10,"thpercentile":90,"height":10,"summeth":1},"annotation":["11101","22013","30003"],"details":{"cell":"H1-hESC","cell organism":"Human","cell description":"Human Embryonic Stem Cells","cell karyotype":"normal","cell lineage":"embryonic stem cells","cell sex":"M","antibody":"H3K9ac","antibody description":"rabbit polyclonal","antibody target description":"Histone H3 (acetyl K9). 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Antibody Target: RFX5","antibody targetdescription":"Activates transcription from class II MHC promoters. Recognizes X-boxes. Mediates cooperative binding betweenRFX and NF-Y. 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Imposes pluripotency on ES cells and prevents their differentiation towards extraembryonic endoderm and trophectoderm lineages.  Blocks bone morphogenetic protein-induced mesoderm differentiation of ES cells by physically interacting with SMAD1 and interfering with the recruitment of coactivators to the active SMAD transcriptional complexes (By similarity).  Acts as a transcriptional activator or repressor (By similarity).  Binds optimally to the DNA consensus sequence 5'-TAAT[GT][GT]-3' or 5'-[CG][GA][CG]C[GC]ATTAN[GC]-3' (By similarity).  When overexpressed, promotes cells to enter into S phase and proliferation (By similarity) (provided by RefSeq).","antibody vendorname":"Santa Cruz Biotech","antibody vendorid":"sc-33759","treatment":"None","treatment description":"No special treatment or protocol applies","protocol":"v041610.2","protocol description":"2x10^7 cells, fragmentation by probe-in sonicator, one 15-cycle round of PCR (Myers)","controlid":"SL1398","labexpid":"SL2687","replicate":"1","replicate description":"tier 1","original_filename":"GSM803437_hg19_wgEncodeHaibTfbsH1hescNanogsc33759V0416102RawRep1.bigWig"},"geolst":["GSM803437"]},{"type":"bigWig","name":"Stanford ChipSeq H1-hESC GTF2F1","url":"http://egg.wustl.edu/d/hg19/GSM935581_1.bigWig","public":true,"mode":1,"qtc":{"anglescale":1,"pr":0,"pg":0,"pb":230,"nr":255,"ng":0,"nb":0,"pth":"#ffff00","nth":"#800000","thtype":0,"thmin":0,"thmax":10,"thpercentile":90,"height":10,"summeth":1},"annotation":["11101","24651","30018"],"details":{"lab":"Stanford","lab description":"Snyder - Stanford University","datatype":"ChipSeq","datatype description":"Chromatin IP Sequencing","cell":"H1-hESC","cell organism":"human","cell description":"embryonic stem cells","cell karyotype":"normal","cell lineage":"inner cell mass","cell sex":"M","treatment":"None","treatment description":"No special treatment or protocol applies","antibody":"GTF2F1_(AB28179)","antibody antibodydescription":"Rabbit polyclonal to TFIIF - Recombinant full length protein - RAP 74 subunit of TF11F. 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When overexpressed, promotes cells to enter into S phase and proliferation (By similarity) (provided by RefSeq).","antibody vendorname":"Santa Cruz Biotech","antibody vendorid":"sc-33759","treatment":"None","treatment description":"No special treatment or protocol applies","protocol":"v041610.2","protocol description":"2x10^7 cells, fragmentation by probe-in sonicator, one 15-cycle round of PCR (Myers)","controlid":"SL1398","labexpid":"SL2687","replicate":"1","replicate description":"tier 1","original_filename":"GSM803437_hg19_wgEncodeHaibTfbsH1hescNanogsc33759V0416102RawRep2.bigWig"},"geolst":["GSM803437"]},{"type":"bigWig","name":"HudsonAlpha ChipSeq H1-hESC TAF7","url":"http://egg.wustl.edu/d/hg19/GSM803501_2.bigWig","public":true,"mode":1,"qtc":{"anglescale":1,"pr":0,"pg":0,"pb":230,"nr":255,"ng":0,"nb":0,"pth":"#ffff00","nth":"#800000","thtype":0,"thmin":0,"thmax":10,"thpercentile":90,"height":10,"summeth":1},"annotation":["11101","24046","30015"],"details":{"lab":"HudsonAlpha","lab description":"Myers - Hudson Alpha Institute for Biotechnology","datatype":"ChipSeq","datatype description":"ChIP-Seq","cell":"H1-hESC","cell organism":"human","cell description":"embryonic stem cells","cell karyotype":"normal","cell lineage":"inner cell mass","cell sex":"M","antibody":"TAF7_(SC-101167)","antibody antibodydescription":"Mouse monoclonal IgG, raised against recombinant TAF II p55 (TAF7) of human origin.","antibody targetdescription":"The intronless gene for this transcription coactivator is located between the protocadherin beta and gamma gene clusters on chromosome 5. 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The protein is required for transcription by promoters targeted by RNA polymerase II (RefSeq).","antibody vendorname":"Santa Cruz Biotech","antibody vendorid":"sc-101167","treatment":"None","treatment description":"No special treatment or protocol applies","protocol":"v041610.2","protocol description":"2x10^7 cells, fragmentation by probe-in sonicator, one 15-cycle round of PCR (Myers)","controlid":"SL1398","labexpid":"SL2697","replicate":"1","replicate description":"tier 1","original_filename":"GSM803501_hg19_wgEncodeHaibTfbsH1hescTaf7sc101167V0416102RawRep2.bigWig"},"geolst":["GSM803501"]},{"type":"bigWig","name":"HudsonAlpha ChipSeq H1-hESC TAF7","url":"http://egg.wustl.edu/d/hg19/GSM803501_1.bigWig","public":true,"mode":1,"qtc":{"anglescale":1,"pr":0,"pg":0,"pb":230,"nr":255,"ng":0,"nb":0,"pth":"#ffff00","nth":"#800000","thtype":0,"thmin":0,"thmax":10,"thpercentile":90,"height":10,"summeth":1},"annotation":["11101","24046","30015"],"details":{"lab":"HudsonAlpha","lab description":"Myers - Hudson Alpha Institute for Biotechnology","datatype":"ChipSeq","datatype description":"ChIP-Seq","cell":"H1-hESC","cell organism":"human","cell description":"embryonic stem cells","cell karyotype":"normal","cell lineage":"inner cell mass","cell sex":"M","antibody":"TAF7_(SC-101167)","antibody antibodydescription":"Mouse monoclonal IgG, raised against recombinant TAF II p55 (TAF7) of human origin.","antibody targetdescription":"The intronless gene for this transcription coactivator is located between the protocadherin beta and gamma gene clusters on chromosome 5. 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California Institute of Technology","datatype":"RnaSeq","datatype description":"Sequencing analysis of RNA expression","cell":"H1-hESC","cell organism":"human","cell description":"embryonic stem cells","cell karyotype":"normal","cell lineage":"inner cell mass","cell sex":"M","readtype":"1x75D","readtype description":"Single 75 nt directed reads","insertlength":"None","insertlength description":"No special treatment or protocol applies","treatment":"None","treatment description":"No special treatment or protocol applies","labexpid":"10947","mapalgorithm":"TH1014","mapalgorithm description":"TopHat v1.0.14","replicate":"1","original_filename":"GSM958737_hg19_wgEncodeCaltechRnaSeqH1hescR1x75dTh1014UMinusRawRep2V4.bigWig"},"geolst":["GSM958737"]},{"type":"bigWig","name":"HudsonAlpha ChipSeq H1-hESC TCF12","url":"http://egg.wustl.edu/d/hg19/GSM803427_2.bigWig","public":true,"mode":1,"qtc":{"anglescale":1,"pr":0,"pg":0,"pb":230,"nr":255,"ng":0,"nb":0,"pth":"#ffff00","nth":"#800000","thtype":0,"thmin":0,"thmax":10,"thpercentile":90,"height":10,"summeth":1},"annotation":["11101","24015","30015"],"details":{"lab":"HudsonAlpha","lab description":"Myers - 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Several alternatively spliced transcript variants of this gene have been described, but the full-length nature of some of these variants has not been determined.","antibody vendorname":"Santa Cruz Biotech","antibody vendorid":"sc-357","treatment":"None","treatment description":"No special treatment or protocol applies","protocol":"PCR1x","protocol description":"1-cycle of PCR (Myers)","controlid":"SL1398","labexpid":"SL1158","replicate":"1","replicate description":"tier 1","original_filename":"GSM803427_hg19_wgEncodeHaibTfbsH1hescTcf12Pcr1xRawRep2.bigWig"},"geolst":["GSM803427"]},{"type":"bigWig","name":"Stanford ChipSeq H1-hESC CHD2","url":"http://egg.wustl.edu/d/hg19/GSM935297_1.bigWig","public":true,"mode":1,"qtc":{"anglescale":1,"pr":0,"pg":0,"pb":230,"nr":255,"ng":0,"nb":0,"pth":"#ffff00","nth":"#800000","thtype":0,"thmin":0,"thmax":10,"thpercentile":90,"height":10,"summeth":1},"annotation":["11101","24606","30018"],"details":{"lab":"Stanford","lab description":"Snyder - Stanford University","datatype":"ChipSeq","datatype description":"Chromatin IP Sequencing","cell":"H1-hESC","cell organism":"human","cell description":"embryonic stem cells","cell karyotype":"normal","cell lineage":"inner cell mass","cell sex":"M","treatment":"None","treatment description":"No special treatment or protocol applies","antibody":"CHD2_(AB68301)","antibody antibodydescription":"Rabbit polyclonal to CHD2 Synthetic peptide derived from within residues 1250 - 1350 of Human CHD2. Antibody Target: CHD2","antibody targetdescription":"CHD family of proteins are characterized by presence of chromo (chromatin organization modifier) domains and SNF2-related helicase/ATPase domains. CHD genes alter gene expression possibly by modification of chromatin structure thus altering access of the transcriptional apparatus to its chromosomal DNA template.","antibody vendorname":"Abcam","antibody vendorid":"ab68301","control":"IgG-rab","control description":"Input signal from Normal Rabbit IgG ChIP-seq.","controlid":"wgEncodeEH001834","replicate":"1","original_filename":"GSM935297_hg19_wgEncodeSydhTfbsH1hescChd2IggrabSig.bigWig"},"geolst":["GSM935297"]},{"type":"bigWig","name":"Stanford ChipSeq H1-hESC CEBPB","url":"http://egg.wustl.edu/d/hg19/GSM935295_1.bigWig","public":true,"mode":1,"qtc":{"anglescale":1,"pr":0,"pg":0,"pb":230,"nr":255,"ng":0,"nb":0,"pth":"#ffff00","nth":"#800000","thtype":0,"thmin":0,"thmax":10,"thpercentile":90,"height":10,"summeth":1},"annotation":["11101","24624","30018"],"details":{"lab":"Stanford","lab description":"Snyder - Stanford University","datatype":"ChipSeq","datatype description":"Chromatin IP Sequencing","cell":"H1-hESC","cell organism":"human","cell description":"embryonic stem cells","cell karyotype":"normal","cell lineage":"inner cell mass","cell sex":"M","treatment":"None","treatment description":"No special treatment or protocol applies","antibody":"CEBPB","antibody antibodydescription":"Rabbit polyclonal IgG. Antibody Target: CEBPB","antibody targetdescription":"epitope mapping at the C-terminus of C/EBP-beta of rat origin","antibody vendorname":"Santa Cruz Biotech","antibody vendorid":"sc-150","control":"IgG-rab","control description":"Input signal from Normal Rabbit IgG ChIP-seq.","controlid":"wgEncodeEH001834","replicate":"1","original_filename":"GSM935295_hg19_wgEncodeSydhTfbsH1hescCebpbIggrabSig.bigWig"},"geolst":["GSM935295"]},{"type":"bigWig","name":"Broad ChipSeq H1-hESC EZH2","url":"http://egg.wustl.edu/d/hg19/GSM1003524_1.bigWig","public":true,"mode":1,"qtc":{"anglescale":1,"pr":0,"pg":0,"pb":230,"nr":255,"ng":0,"nb":0,"pth":"#ffff00","nth":"#800000","thtype":0,"thmin":0,"thmax":10,"thpercentile":90,"height":10,"summeth":1},"annotation":["11101","22060","30003"],"details":{"datatype":"ChipSeq","datatype description":"Chromatin IP Sequencing","antibody antibodydescription":"This EZH2 antibody was raised against a recombinant protein corresponding to amino acids 353-451 of human EZH2. Antibody Target: EZH2","antibody targetdescription":"EZH2 (Enhancer of Zeste homolog 2, also designated Enx1 and SETDB1) is a human homolog of the Drosophila Polycomb-group protein Enhancer of Zeste protein. It contains a SET domain that catalyzes the methylation of histone H3 at lysine 27 (Histone H3 Lys27 monomethylated, dimethylated and trimethylated). Polycomb-group proteins repress gene expression by binding to chromatin and locally altering chromatin structure. EZH2, BMI-1 and Suz12 are present in the PRC2 and PRC3 protein complexes that function as mediators of epigenetic transcriptional silencing. EZH2 associates with the embryonic ectoderm development protein, the VAV1 oncoprotein, and the X-linked nuclear protein. EZH2 may play a role in the hematopoietic and central nervous systems. Deregulation of EZH2 is linked to a variety of cancers.","antibody vendorname":"Active Motif","antibody vendorid":"39875","controlid":"H1-hESC/None/Input/std","replicate":"1,2","softwareversion":"ScriptureVPaperR3","cell sex":"M","antibody":"EZH2_(39875)","treatment":"None","treatment description":"No special treatment or protocol applies","control":"std","control description":"Standard input signal for most experiments.","labversion":"Illumina_HiSeq_2000","labversion description":"Illumina HiSeq 2000","original_filename":"GSM1003524_hg19_wgEncodeBroadHistoneH1hescEzh239875Sig.bigWig"},"geolst":["GSM1003524"]},{"type":"bigWig","name":"HudsonAlpha ChipSeq H1-hESC Sin3Ak-20","url":"http://egg.wustl.edu/d/hg19/GSM803428_2.bigWig","public":true,"mode":1,"qtc":{"anglescale":1,"pr":0,"pg":0,"pb":230,"nr":255,"ng":0,"nb":0,"pth":"#ffff00","nth":"#800000","thtype":0,"thmin":0,"thmax":10,"thpercentile":90,"height":10,"summeth":1},"annotation":["11101","24054","30015"],"details":{"lab":"HudsonAlpha","lab description":"Myers - Hudson Alpha Institute for Biotechnology","datatype":"ChipSeq","datatype description":"ChIP-Seq","cell":"H1-hESC","cell organism":"human","cell description":"embryonic stem cells","cell karyotype":"normal","cell lineage":"inner cell mass","cell sex":"M","antibody":"Sin3Ak-20","antibody antibodydescription":"Rabbit polyclonal, Sin3A (k-20).","antibody targetdescription":"Co-repressor interacting with HDAC1, N-coR, SMRT, and MeCP2","antibody vendorname":"Santa Cruz Biotech","antibody vendorid":"sc-994","treatment":"None","treatment description":"No special treatment or protocol applies","protocol":"PCR1x","protocol description":"1-cycle of PCR (Myers)","controlid":"SL1398","labexpid":"SL1157","replicate":"1","replicate description":"tier 1","original_filename":"GSM803428_hg19_wgEncodeHaibTfbsH1hescSin3ak20Pcr1xRawRep2.bigWig"},"geolst":["GSM803428"]},{"type":"bigWig","name":"Broad ChipSeq H1-hESC P300","url":"http://egg.wustl.edu/d/hg19/GSM1003513_1.bigWig","public":true,"mode":1,"qtc":{"anglescale":1,"pr":0,"pg":0,"pb":230,"nr":255,"ng":0,"nb":0,"pth":"#ffff00","nth":"#800000","thtype":0,"thmin":0,"thmax":10,"thpercentile":90,"height":10,"summeth":1},"annotation":["11101","24011","30003"],"details":{"datatype":"ChipSeq","datatype description":"Chromatin IP Sequencing","antibody antibodydescription":"Rabbit Polyclonal Antigen Affinity Purified, Unconjugated, Liquid","antibody targetdescription":"Histone acetyltransferase with sequence similarity to CBP (CREB-binding protein). Regulates transcription by influencing chromatin organization. P300 has been demonstrated to bind to phosphorylated CREB to mediate cAMP-gene regulation.","antibody vendorname":"Bethyl","antibody vendorid":"A300-358A","controlid":"H1-hESC/None/Input/std","replicate":"1","softwareversion":"ScriptureVPaperR3","cell sex":"M","antibody":"P300_KAT3B","treatment":"None","treatment description":"No special treatment or protocol applies","control":"std","control description":"Standard input signal for most experiments.","labversion":"Illumina_GA2x","labversion description":"Illumina Genome Analyzer IIx","original_filename":"GSM1003513_hg19_wgEncodeBroadHistoneH1hescP300kat3bSig.bigWig"},"geolst":["GSM1003513"]},{"type":"bigWig","name":"Stanford ChipSeq H1-hESC Znf143","url":"http://egg.wustl.edu/d/hg19/GSM935514_1.bigWig","public":true,"mode":1,"qtc":{"anglescale":1,"pr":0,"pg":0,"pb":230,"nr":255,"ng":0,"nb":0,"pth":"#ffff00","nth":"#800000","thtype":0,"thmin":0,"thmax":10,"thpercentile":90,"height":10,"summeth":1},"annotation":["11101","24616","30018"],"details":{"lab":"Stanford","lab description":"Snyder - Stanford University","datatype":"ChipSeq","datatype description":"Chromatin IP Sequencing","cell":"H1-hESC","cell organism":"human","cell description":"embryonic stem cells","cell karyotype":"normal","cell lineage":"inner cell mass","cell sex":"M","treatment":"None","treatment description":"No special treatment or protocol applies","antibody":"Znf143_(16618-1-AP)","antibody antibodydescription":"Rabbit polyclonal against human ZNF143 (amino acids 283-626)-6his fusion protein. Antibody Target: Znf143","antibody targetdescription":"Transcriptional activator. Activates the gene for selenocysteine tRNA (tRNAsec). Binds to the SPH motif of small nuclear RNA (snRNA) gene promoters. Participates to efficient U6 RNA polymerase III transcription via its interaction with CHD8","antibody vendorname":"Proteintech","antibody vendorid":"16618-1-AP","control":"IgG-rab","control description":"Input signal from Normal Rabbit IgG ChIP-seq.","controlid":"wgEncodeEH001834","replicate":"1","original_filename":"GSM935514_hg19_wgEncodeSydhTfbsH1hescZnf143IggrabSig.bigWig"},"geolst":["GSM935514"]},{"type":"bigWig","name":"H1 RBBP5 1","url":"http://egg.wustl.edu/d/hg19/GSM831038_1.bigWig","public":true,"mode":1,"qtc":{"anglescale":1,"pr":0,"pg":0,"pb":230,"nr":255,"ng":0,"nb":0,"pth":"#ffff00","nth":"#800000","thtype":0,"thmin":0,"thmax":10,"thpercentile":90,"height":10,"summeth":1},"annotation":["11101","22040","30003"],"details":{"cell type":"H1 ES cells were grown in TeSR media on Matrigel (Cellular Dynamics)","chip antibody":"Bethyl,A300.109A","original_filename":"GSM831038_H1_RBBP5_1.bw"},"geolst":["GSM831038"]},{"type":"bigWig","name":"HudsonAlpha ChipSeq H1-hESC E2F6","url":"http://egg.wustl.edu/d/hg19/GSM1010899_2.bigWig","public":true,"mode":1,"qtc":{"anglescale":1,"pr":0,"pg":0,"pb":230,"nr":255,"ng":0,"nb":0,"pth":"#ffff00","nth":"#800000","thtype":0,"thmin":0,"thmax":10,"thpercentile":90,"height":10,"summeth":1},"annotation":["11101","24060","30015"],"details":{"datatype":"ChipSeq","datatype description":"Chromatin IP Sequencing","antibody antibodydescription":"Rabbit polyclonal IgG, epitope corresponding to a.a. 232-281 mapping at the C-terminus of E2F-6 of human origin. Antibody Target: E2F6","antibody targetdescription":"This gene encodes a member of the E2F transcription factor protein family. E2F family members play a crucial role in control of the cell cycle and of the action of tumor suppressor proteins. They are also a target of the transforming proteins of small DNA tumor viruses. Many E2F proteins contain several evolutionarily conserved domains: a DNA binding domain, a dimerization domain which determines interaction with the differentiation regulated transcription factor proteins (DP), a transactivation domain enriched in acidic amino acids, and a tumor suppressor protein association domain which is embedded within the transactivation domain. The encoded protein of this gene is atypical because it lacks the transactivation and tumor suppressor protein association domains. It contains a modular suppression domain and is an inhibitor of E2F-dependent transcription. The protein is part of a multimeric protein complex that contains a histone methyltransferase and the transcription factors Mga and Max. Multiple transcript variants have been reported for this gene, but it has not been clearly demonstrated that they encode valid isoforms (RefSeq).","antibody vendorname":"Santa Cruz Biotechnology","antibody vendorid":"sc-22823","controlid":"SL12796","labexpid":"SL13304,SL12414","softwareversion":"MACS","cell sex":"M","antibody":"E2F6","treatment":"None","treatment description":"No special treatment or protocol applies","protocol":"v042211.1","protocol description":"Faster ChIP protocol & AMpure XP size selection for ChIP-seq (Myers)","replicate":"1,2","original_filename":"GSM1010899_hg19_wgEncodeHaibTfbsH1hescE2f6V0422111RawRep2.bigWig"},"geolst":["GSM1010899"]},{"type":"bigWig","name":"Broad ChipSeq H1-hESC SIRT6","url":"http://egg.wustl.edu/d/hg19/GSM1003451_1.bigWig","public":true,"mode":1,"qtc":{"anglescale":1,"pr":0,"pg":0,"pb":230,"nr":255,"ng":0,"nb":0,"pth":"#ffff00","nth":"#800000","thtype":0,"thmin":0,"thmax":10,"thpercentile":90,"height":10,"summeth":1},"annotation":["11101","22050","30003"],"details":{"datatype":"ChipSeq","datatype description":"Chromatin IP Sequencing","antibody antibodydescription":"Rabbit polyclonal. Antibody Target: SIRT6","antibody targetdescription":"A synthetic peptide made to an internal region of the human SIRT6 protein","antibody vendorname":"Abcam","antibody vendorid":"ab48192","controlid":"H1-hESC/None/Input/std","replicate":"1","softwareversion":"ScriptureVPaperR3","cell sex":"M","antibody":"SIRT6","treatment":"None","treatment description":"No special treatment or protocol applies","control":"std","control description":"Standard input signal for most experiments.","labversion":"Illumina_GA2x","labversion description":"Illumina Genome Analyzer IIx","original_filename":"GSM1003451_hg19_wgEncodeBroadHistoneH1hescSirt6Sig.bigWig"},"geolst":["GSM1003451"]},{"type":"bigWig","name":"Broad ChipSeq H1-hESC PLU1","url":"http://egg.wustl.edu/d/hg19/GSM1003457_1.bigWig","public":true,"mode":1,"qtc":{"anglescale":1,"pr":0,"pg":0,"pb":230,"nr":255,"ng":0,"nb":0,"pth":"#ffff00","nth":"#800000","thtype":0,"thmin":0,"thmax":10,"thpercentile":90,"height":10,"summeth":1},"annotation":["11101","22039","30003"],"details":{"datatype":"ChipSeq","datatype description":"Chromatin IP Sequencing","antibody antibodydescription":"Polyclonal Antigen Affinity Purified, Unconjugated, Liquid. Antibody Target: PLU1","antibody targetdescription":"JARID1B (Jumonji AT-rich interactive domain 1B) is a member of the JARID 1 family of proteins that catalyze the demethylation of histones on lysine. The Jarid 1 family members include JARID1A, JARID1B, JARID1C and JARID1D. The members of this family possess a Jumonji C (JmjC) and Jumonji N (JmjN) domain as well as one ARID (AT-rich interactive domain) and three PHD-type zinc fingers. As histone demethylases, JARID 1 proteins function as transcriptional repressors. JARID1B expression is largely restricted to testis and may play a critical role in tumorigenesis as it has been found to be upregulated in prostate and breast cancer. Alternative names for JARID1B include retinoblastoma-binding protein 2 homolog 1, RBP2-H1, cancer/testis antigen 31, CT31, PLU-1, PUT1, and KDM5B.","antibody vendorname":"YangShi lab","antibody vendorid":"PLU1","controlid":"H1-hESC/None/Input/std","replicate":"1","softwareversion":"ScriptureVPaperR3","cell sex":"M","antibody":"PLU1","treatment":"None","treatment description":"No special treatment or protocol applies","control":"std","control description":"Standard input signal for most experiments.","labversion":"Illumina_GA2x","labversion description":"Illumina Genome Analyzer IIx","original_filename":"GSM1003457_hg19_wgEncodeBroadHistoneH1hescPlu1Sig.bigWig"},"geolst":["GSM1003457"]},{"type":"bigWig","name":"RIKEN Cage H1-hESC cell","url":"http://egg.wustl.edu/d/hg19/GSM849357_4.bigWig","public":true,"mode":1,"qtc":{"anglescale":1,"pr":0,"pg":0,"pb":230,"nr":255,"ng":0,"nb":0,"pth":"#ffff00","nth":"#800000","thtype":0,"thmin":0,"thmax":10,"thpercentile":90,"height":10,"summeth":1},"annotation":["11101","25004","30011"],"details":{"lab":"RIKEN","lab description":"Carninci - RIKEN Omics Science Center","datatype":"Cage","datatype description":"CAGE","cell":"H1-hESC","cell organism":"human","cell description":"embryonic stem cells","cell karyotype":"normal","cell lineage":"inner cell mass","cell sex":"M","localization":"cell","localization description":"Whole cell","rnaextract":"longPolyA","rnaextract description":"Poly(A)+ RNA longer than 200 nt","biorep":"005WC","labexpid":"J74","replicate":"1","replicate description":"tier 1","original_filename":"GSM849357_hg19_wgEncodeRikenCageH1hescCellPapPlusSignalRep2.bigWig"},"geolst":["GSM849357"]},{"type":"bigWig","name":"HudsonAlpha ChipSeq H1-hESC POU5F1","url":"http://egg.wustl.edu/d/hg19/GSM803438_1.bigWig","public":true,"mode":1,"qtc":{"anglescale":1,"pr":0,"pg":0,"pb":230,"nr":255,"ng":0,"nb":0,"pth":"#ffff00","nth":"#800000","thtype":0,"thmin":0,"thmax":10,"thpercentile":90,"height":10,"summeth":1},"annotation":["11101","24056","30015"],"details":{"lab":"HudsonAlpha","lab description":"Myers - Hudson Alpha Institute for Biotechnology","datatype":"ChipSeq","datatype description":"ChIP-Seq","cell":"H1-hESC","cell organism":"human","cell description":"embryonic stem cells","cell karyotype":"normal","cell lineage":"inner cell mass","cell sex":"M","antibody":"POU5F1_(SC-9081)","antibody antibodydescription":"Rabbit polyclonal IgG, epitope corresponding to amino acids 1-134 of Oct3/4 of human origin.","antibody targetdescription":"This gene encodes a transcription factor containing a POU homeodomain.  This transcription factor plays a role in embryonic development, especially during early embryogenesis, and it is necessary for embryonic stem cell pluripotency.  A translocation of this gene with the Ewing's sarcoma gene, t(6;22)(p21;q12), has been linked to tumor formation.  Alternative splicing, as well as usage of alternative translation initiation codons, results in multiple isoforms, one of which initiates at a non-AUG (CUG) start codon.  Related pseudogenes have been identified on chromosomes 1, 3, 8, 10, and 12 (provided by RefSeq).","antibody vendorname":"Santa Cruz Biotech","antibody vendorid":"sc-9081","treatment":"None","treatment description":"No special treatment or protocol applies","protocol":"v041610.2","protocol description":"2x10^7 cells, fragmentation by probe-in sonicator, one 15-cycle round of PCR (Myers)","controlid":"SL1398","labexpid":"SL2548","replicate":"1","replicate description":"tier 1","original_filename":"GSM803438_hg19_wgEncodeHaibTfbsH1hescPou5f1sc9081V0416102RawRep1.bigWig"},"geolst":["GSM803438"]},{"type":"bigWig","name":"Broad ChipSeq H1-hESC RBBP5","url":"http://egg.wustl.edu/d/hg19/GSM1003584_1.bigWig","public":true,"mode":1,"qtc":{"anglescale":1,"pr":0,"pg":0,"pb":230,"nr":255,"ng":0,"nb":0,"pth":"#ffff00","nth":"#800000","thtype":0,"thmin":0,"thmax":10,"thpercentile":90,"height":10,"summeth":1},"annotation":["11101","22040","30003"],"details":{"datatype":"ChipSeq","datatype description":"Chromatin IP Sequencing","antibody antibodydescription":"Polyclonal Antigen Affinity Purified, Unconjugated, Liquid. Antibody Target: RBBP5","antibody targetdescription":"Retinoblastoma binding protein 5 (RbBP5) is a subunit of a human Set1-like histone methyltransferase (HMT) complex. The Set-1 like complexes are responsible for mono-, di-, and tri-methylation of histone H3 K4. RbBP5 associates with a multitude of proteins in a number of Set1-like complexes which include various combinations of MLL, MLL2, MLL3, MLL4, ASH2L,WDR5, hDPY-30, hSwd2, CXXC1, HCF1, Menin, PTIP, PA1, NCOA6, and UTX. RbBP5 is also one of many proteins that bind the Retinoblastoma tumor suppressor protein, pRb. Alternate names for RbBP5 are RBQ3 and SWD1.","antibody vendorname":"Bethyl Laboratories","antibody vendorid":"A300-109A","controlid":"H1-hESC/Input/std","replicate":"1,2","softwareversion":"ScriptureVPaperR3","cell sex":"M","antibody":"RBBP5_(A300-109A)","treatment":"None","treatment description":"No special treatment or protocol applies","control":"std","control description":"Standard input signal for most experiments.","original_filename":"GSM1003584_hg19_wgEncodeBroadHistoneH1hescRbbp5a300109aStdSig.bigWig"},"geolst":["GSM1003584"]},{"type":"bigWig","name":"UT-A ChipSeq H1-hESC Pol2","url":"http://egg.wustl.edu/d/hg19/GSM822300_2.bigWig","public":true,"mode":1,"qtc":{"anglescale":1,"pr":0,"pg":0,"pb":230,"nr":255,"ng":0,"nb":0,"pth":"#ffff00","nth":"#800000","thtype":0,"thmin":0,"thmax":10,"thpercentile":90,"height":10,"summeth":1},"annotation":["11101","24003","30009"],"details":{"lab":"UT-A","lab description":"Iyer - University of Texas at Austin","datatype":"ChipSeq","datatype description":"ChIP-Seq","cell":"H1-hESC","cell organism":"human","cell description":"embryonic stem cells","cell karyotype":"normal","cell lineage":"inner cell mass","cell sex":"M","antibody":"Pol2","antibody antibodydescription":"mouse monoclonal, clone 8WG16","antibody targetdescription":"RNA Polymerase II","antibody vendorname":"Covance","antibody vendorid":"MMS-126R","controlid":"generic_male","labversion":"align_on_cluster_bwa.pl v 1","replicate":"1","replicate description":"tier 1","original_filename":"GSM822300_hg19_wgEncodeOpenChromChipH1hescPol2Sig.bigWig"},"geolst":["GSM822300"]},{"type":"bigWig","name":"HudsonAlpha ChipSeq H1-hESC E2F6","url":"http://egg.wustl.edu/d/hg19/GSM1010899_1.bigWig","public":true,"mode":1,"qtc":{"anglescale":1,"pr":0,"pg":0,"pb":230,"nr":255,"ng":0,"nb":0,"pth":"#ffff00","nth":"#800000","thtype":0,"thmin":0,"thmax":10,"thpercentile":90,"height":10,"summeth":1},"annotation":["11101","24060","30015"],"details":{"datatype":"ChipSeq","datatype description":"Chromatin IP Sequencing","antibody antibodydescription":"Rabbit polyclonal IgG, epitope corresponding to a.a. 232-281 mapping at the C-terminus of E2F-6 of human origin. Antibody Target: E2F6","antibody targetdescription":"This gene encodes a member of the E2F transcription factor protein family. E2F family members play a crucial role in control of the cell cycle and of the action of tumor suppressor proteins. They are also a target of the transforming proteins of small DNA tumor viruses. Many E2F proteins contain several evolutionarily conserved domains: a DNA binding domain, a dimerization domain which determines interaction with the differentiation regulated transcription factor proteins (DP), a transactivation domain enriched in acidic amino acids, and a tumor suppressor protein association domain which is embedded within the transactivation domain. The encoded protein of this gene is atypical because it lacks the transactivation and tumor suppressor protein association domains. It contains a modular suppression domain and is an inhibitor of E2F-dependent transcription. The protein is part of a multimeric protein complex that contains a histone methyltransferase and the transcription factors Mga and Max. Multiple transcript variants have been reported for this gene, but it has not been clearly demonstrated that they encode valid isoforms (RefSeq).","antibody vendorname":"Santa Cruz Biotechnology","antibody vendorid":"sc-22823","controlid":"SL12796","labexpid":"SL13304,SL12414","softwareversion":"MACS","cell sex":"M","antibody":"E2F6","treatment":"None","treatment description":"No special treatment or protocol applies","protocol":"v042211.1","protocol description":"Faster ChIP protocol & AMpure XP size selection for ChIP-seq (Myers)","replicate":"1,2","original_filename":"GSM1010899_hg19_wgEncodeHaibTfbsH1hescE2f6V0422111RawRep1.bigWig"},"geolst":["GSM1010899"]},{"type":"bigWig","name":"Broad ChipSeq H1-hESC H3K9me3","url":"http://egg.wustl.edu/d/hg19/GSM1003585_1.bigWig","public":true,"mode":1,"qtc":{"anglescale":1,"pr":159,"pg":0,"pb":72,"nr":255,"ng":0,"nb":0,"pth":"#ffff00","nth":"#800000","thtype":0,"thmin":0,"thmax":10,"thpercentile":90,"height":10,"summeth":1},"annotation":["11101","22012","30003"],"details":{"datatype":"ChipSeq","datatype description":"Chromatin IP Sequencing","antibody antibodydescription":"rabbit polyclonal, ChIP grade. Antibody Target: H3K9me3","antibody targetdescription":"Histone H3 (tri methyl K9). Is associated with repressive heterochromatic state (silenced chromatin). NOTE CONTRAST to H3K9me1 which is associated with active and accessible regions.","antibody vendorname":"Abcam","antibody vendorid":"ab8898","controlid":"H1-hESC/Input/std","replicate":"1,2","softwareversion":"ScriptureVPaperR3","cell sex":"M","antibody":"H3K9me3","treatment":"None","treatment description":"No special treatment or protocol applies","control":"std","control description":"Standard input signal for most experiments.","original_filename":"GSM1003585_hg19_wgEncodeBroadHistoneH1hescH3k09me3StdSig.bigWig"},"geolst":["GSM1003585"]},{"type":"bigWig","name":"Stanford ChipSeq H1-hESC BRCA1","url":"http://egg.wustl.edu/d/hg19/GSM935517_1.bigWig","public":true,"mode":1,"qtc":{"anglescale":1,"pr":0,"pg":0,"pb":230,"nr":255,"ng":0,"nb":0,"pth":"#ffff00","nth":"#800000","thtype":0,"thmin":0,"thmax":10,"thpercentile":90,"height":10,"summeth":1},"annotation":["11101","24631","30018"],"details":{"lab":"Stanford","lab description":"Snyder - Stanford University","datatype":"ChipSeq","datatype description":"Chromatin IP Sequencing","cell":"H1-hESC","cell organism":"human","cell description":"embryonic stem cells","cell karyotype":"normal","cell lineage":"inner cell mass","cell sex":"M","treatment":"None","treatment description":"No special treatment or protocol applies","antibody":"BRCA1_(A300-000A)","antibody antibodydescription":"Rabbit polyclonal recognizing a region between residue 1800 and the C-terminus (residue 1863) of human Breast Cancer Gene 1. Antibody Target: BRCA1","antibody targetdescription":"Nuclear phosphoprotein that plays a role in maintaining genomic stability, and acts as a tumor suppressor. Associates with RNA polymerase II, and through the C-terminal domain, also interacts with histone deacetylase complexes. Plays a role in transcription, DNA repair of double-stranded breaks, and recombination. Mutations in this gene are responsible for approximately 40% of inherited breast cancers and more than 80% of inherited breast and ovarian cancers.","antibody vendorname":"Bethyl Labratories","antibody vendorid":"A300-000A","control":"IgG-rab","control description":"Input signal from Normal Rabbit IgG ChIP-seq.","controlid":"wgEncodeEH001834","replicate":"1","original_filename":"GSM935517_hg19_wgEncodeSydhTfbsH1hescBrca1IggrabSig.bigWig"},"geolst":["GSM935517"]},{"type":"bigWig","name":"CSHL RnaSeq H1-hESC cytosol","url":"http://egg.wustl.edu/d/hg19/GSM897072_2.bigWig","public":true,"mode":1,"qtc":{"anglescale":1,"pr":0,"pg":0,"pb":230,"nr":255,"ng":0,"nb":0,"pth":"#ffff00","nth":"#800000","thtype":0,"thmin":0,"thmax":10,"thpercentile":90,"height":10,"summeth":1},"annotation":["11101","25002","30006"],"details":{"lab":"CSHL","lab description":"Gingeras - Cold Spring Harbor Laboratory","datatype":"RnaSeq","datatype description":"Sequencing analysis of RNA expression","cell":"H1-hESC","cell organism":"human","cell description":"embryonic stem cells","cell karyotype":"normal","cell lineage":"inner cell mass","cell sex":"M","localization":"cytosol","localization description":"The fluid between the cells outer membrane and the nucleus","protocol":"TAP-Only","protocol description":"This kind of library was made from RNA < 200 nucleotides that were pre-treated with Tobacco Acid Pyrophosphatase to remove any 5' caps that would preclude cloning. 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